video 1	video 2	video 3
video 4	video 5

Video 1: KFP-formation in the periphery of EK18-1-cells (see corresponding boxed area in Fig. 1A)

Time-lapse fluorescence recording (projection of 13 focal image planes) of an EK18-1 cell producing fluorescent HK18-YPF protein chimera. Note the abundant amount of emerging KFPs in the cell periphery, which are transported towards the cell centre. The display rate is 10 frames/s, and images were taken every minute.

Video 2: KFP-formation in the periphery of confluent EK18-1 cells.

The confocal time lapse recording of 5 projected fluorescence images (inverse presentation; overview at left; high power views of boxed areas at right) reveals ongoing KFP formation close to the plasma membrane of tightly attached cells. The display rate is 15 frames/s, and images were taken every 90 seconds.

Video 3: Microtubule-independent inward transport of KFPs (see corresponding Fig. 1A, B).

Fluorescence recording of HK18-YFP in a peripheral region of an EK18-1 cell treated with 100 µM nocodazole reveals continuing inward movement of newly-formed KFPs. Images were acquired every 20 s and the inhibitor was added at 18 min. The display rate is 10 frames/s.

Video 4: Need of intact actin filaments for inward-directed transport of newly synthesized keratin particles (see corresponding Fig. 1C, D).

Time-lapse movie of the cell edge of HK18-YFP fluorescence in an EK18-1 cell treated with the actin-polymerisation inhibitor cytochalasin D (1 µM). Images were taken every 20 s and the inhibitor was added at 18 min. Note the abrupt halt of KFP transport and the accumulation of KFPs in the cell periphery upon drug administration. The display rate is 10 frames/s.

Video 5: Animated model of keratin filament precursor formation and transport (see corresponding Fig. 4).

The movie schematically shows the peripheral cytoplasm with an extending lamellipodium and depicts successive stages of cytoskeletal rearrangements in relation to focal adhesion formation. Initially, the lamellipodial cell edge is driven outward by cortical actin polymerization. Focal adhesion formation and maturation ensues followed by actin stress fibre anchorage.  Then, KFPs appear at or near focal adhesion sites. Keratin particles enlarge and translocate along actin stress fibres toward the cell interior until integration into the peripheral KF network. For further details see Discussion. Microtubules are depicted in yellow. The display rate is 20 frames/s.