video 1	video 2	video 3
video 4	video 5	video 6
video 7	video 8                           video 9	video 10

Video 1
Tableau of time-lapse recordings (4 frames/min; display rate 10 frames/s) of phase contrast images (upper right) and fluorescence micrographs of a mammary epithelium-derived EpH4 cell that was transfected with cDNAs encoding HK18-YFP and actin-RFP (merged fluorescence images at lower right). Note the extension of an actin-rich lamellipodium and the subsequent formation of a new KF network in this region. See also corresponding Fig. 1 A.

Video 2
Tableau of phase contrast and epifluorescence recordings of an EpH4 cell producing fluorescent chimeras HK18-YFP and actin-RFP. Images were acquired every 15 s and are displayed at 10 frames/s. Note the continuous centripetal movement of KFPs along actin stress fibers in the lamellipodium. See also corresponding Fig. 1 B.

Video 3
Time-lapse fluorescence microscopy of HK18-YFP in an EpH4 cell that was treated with the actin polymerization inhibitor latrunculin B (10 µM). Images were taken every 20 s (display rate 10 frames/s). Note that addition of the drug prevents continuous inward-directed transport of KFPs but that new KFPs continue to appear, elongate and fuse. See also corresponding Fig. 1 D.

Video 4
Tableau of phase contrast and fluorescence micrographs depicting the distribution of HK18-YFP and RFP-zyxin in a lamellipodium of a cDNA-transfected EpH4 cell (merged fluorescence images at lower right). Pictures were recorded every 15 s and are displayed at 10 frames/s. See also corresponding Fig. 2 A.

Video 5
Tableau of phase contrast and fluorescence micrographs depicting the distribution of HK18-YFP and paxillin-DsRed2 in a cDNA-transfected EpH4 cell (merged fluorescence images at upper right). Pictures were recorded every 15 s (display rate 10 frames/s). Note the successive appearance of paxillin-labeled FAs and KFPs in the emerging lamellipodium. See also corresponding Fig. 2 D.

Video 6
Tableau of phase contrast and fluorescence micrographs depicting the distribution of HK18-YFP and RFP-zyxin in a cDNA-transfected SK8/18-2 cell (merged fluorescence images at bottom). Pictures were recorded every 15 s for 50 min (display rate 10 frames/s). Corresponding Fig. 3 A.

Video 7
4D representation of fluorescence time-lapse recording of another region of the SK8/18-2 cell shown in Video 6 depicting HK18-YFP-positive KFs and RFP-zyxin-labeled FAs (correponding Fig. 3 B; recording rate 1 image/15s; display rate 25 frames/s). The red and green axes represent the image plane while the yellow axis corresponds to the time axis. The fluorescence of RFP-zyxin is shown in voxel representation resulting in orange rods that change only little over time as a consequence of the static nature of the FAs. Four KFPs are color-coded to highlight their dynamic behavior in relation to FAs.

Video 8
High magnification images showing fluorescence emitted by RFP-zyxin and HK18-YFP keratin-YFP in a SK8/18-2 cell (corresponding Fig. 3 C; recording rate 1 image/15s; display rate 25 frames/s). Note the appearance, growth and mobility of KFPs in relation to FAs. The cell margin is at top.

Video 9
Time-lapse recording (4 frames/min) of MCF7 cells expressing mutant keratin EYFP-K14R125C and paxillin-DsRed2. Images were acquired every 15 s and are displayed at 25 frames/s. The data are also presented in Fig. 4 A.

Video 10
Double fluorescence recordings of either HK14-YFP or HK14R125C-YFP together with RFP-zyxin in the peripheral cytoplasm of EpH4 cells. Images were recorded every 15 s (display rate 25 frames/s). Note the emergence of KFPs from zyxin-positive structures in both instances.