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movie 1. Time-lapse
epifluorescence microscopy of confluent PK18-5 cells depicting
continuous inward flow of keratin filament fluorescence from the entire
cell circumference. Recording intervals: 30 s. |
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movie 2. Time-lapse
epifluorescence microscopy of peripheral free edge of a PK18-5 cell
showing centripetal flow of HK18-YFP fluorescence originating in a
submembraneous compartment and resulting in integration into the
peripheral filament network. Recording intervals: 30 s. |
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movie 3. Time-lapse
confocal laser scanning microscopy of a peripheral free region of a
human primary keratinocyte depicting the inward-directed aviement of
HK14-YFP fluorescence which originates in a submembraneous compartment
and results in integration of rod-like structures into the peripheral
filament network. Recording intervals: 60 s. |
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movie 4. Time-lapse
confocal laser scanning microscopy of a small peripheral section of a
PK18-5 cell (projection of six focal planes) depicting the formation of
keratin filament precursors below the plasma membrane (position
demarcated by line), their subsequent enlargement and integration into
the filament network. Pictures were taken every 30 s. |
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movie 5. Time-lapse
epifluorescence microscopy of a peripheral region of a SW13 cell
producing fluorescent keratins HK8-CFP and HK18-YFP highlighting
details of keratin filament formation beneath the plasma membrane
(top). Recording of EYFP-elicited fluorescence was every 30 s. |
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movie 6. Time-lapse
fluorescence imaging of a FRAP experiment, in which a section of a
PK18-5 cell producing HK18-YFP was bleached, demonstrating that
filamentous fluorescence recovery occurs preferentially in the cell
periphery. Recording intervals, 2 min. |
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movie 7. Time-lapse
fluorescence image series depicting the distribution of HK13-EGFP in
AK13-1 cells after a short treatment with orthovanadate (10 mM, 4 min).
Recording intervals, 15 s. |
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movie 8. Time-lapse
imaging of HK13-EGFP fluorescence (inverse presentation) in a
peripheral region of an AK13-1 cell in the continued presence of
orthovanadate (10 mM). Images were recorded every 30 s. |
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movie 9. Time-lapse
fluorescence imaging of a peripheral part of a desmosome-free A-431
cells stably synthesizing a dominant-negative connexin-desmoglein
chimera and producing HK13-EGFP after cDNA-transfection. Recording
intervals, 30 s. |
