Abraham C, Bai L, Leube RE. 2011.
Synaptogyrin-dependent modulation of synaptic neurotransmission in Caenorhabditis elegans.
Neuroscience, 190:75-88.
The
tetraspan membrane proteins of the synaptogyrin and synaptophysin type
are abundant and evolutionary conserved synaptic vesicle membrane
proteins whose functions are poorly defined. Their depletion does not
interfere with proper neuronal development and basic neuronal function.
In the search for their function we use the genetic model organism C.
elegans in which, in contrast to vertebrates, the synaptogyrin but not
the synaptophysin orthologue is predominant in neurons. Employing
fluorescent reporter constructs we find that synaptogyrin is expressed
in all GABAergic neurons and most, though not all other neurons.
Subjecting animals either lacking or overexpressing synaptogyrin to the
epileptogenic GABA antagonist pentylenetetrazole reveals increased
sensitivity in comparison to the wild type. Detailed analyses further
uncover mildly altered motility,slightly reduced sensitivity to the
acetylcholine esterase inhibitor aldicarb and decreased recruitment of
synaptobrevin but not of RAB-3 to synapses. Furthermore, synthetic
phenotypes are observed with mutants of the synaptic vesicle recycling
machinery, notably with synaptotagmin, synaptojanin and endophilin
rather than with mutants involved in clathrin-dependent endocytosis.
Taken together, these observations assign a distinct modulatory and
redundant neuronal function to synaptogyrin.
click animated previews to open full size
 |  |  | Movie 1
| Movie 2
| Movie 3
|  |  |  | Movie 4
| Movie 5
| Movie 6
|
Movie 1. Behavior of sng-1(ok234) (BJ14) at a PTZ concentration of 6 mg/ml (corresponding Fig. 1C). Movie 2. Behavior of wild type (BJ20) at a PTZ concentration of 6 mg/ml (corresponding Fig. 1C). Movie 3.
Animation of sng-1p::CFP and unc-47p::GFP fluorescence images recorded
by confocal laser scanning microscopy in the anterior end of a worm of
strain BJ60 kcEx3[sng-1p::cfp]; oxIs12[lin-15(+), unc-47p::gfp],
lin-15(n765ts)X (corresponding Figure 4B anterior). Movie 4.
Animation of sng-1p::CFP and unc-47p::GFP fluorescence images recorded
by confocal laser scanning microscopy in the posterior end of a worm of
strain BJ60 kcEx3[sng-1p::cfp]; oxIs12[lin-15(+), unc-47p::gfp],
lin-15(n765ts)X (corresponding Fig. 4B posterior).
Movie 5.
Animation of fluorescence distribution observed for sng-1p::YFP and DiI
in anterior part of a worm of strain BJ23 kcEx2[sng-1p::yfp] using
confocal laser scanning microscopy (see corresponding Fig. 5Aa, A'a,
A''a). Movie 6.
Animation of fluorescence distribution observed for sng-1p::YFP and DiI
in posterior part of a worm of strain BJ23 kcEx2[sng-1p::yfp] using
confocal laser scanning microscopy (see corresponding Fig. 5Ap, A'p,
A''p).
|
