download.mov (1,3 MB)
download.avi (3 MB)
|
movie 1. Time-lapse
fluorescence microscopy of hepatocellular carcinoma-derived PLC clone
PK18-5 stably expressing fluorescent fusion protein HK18-YFP depicting
the distribution of labeled CKs. The inverse contrast images were
recorded every second and demonstrate details of CKF-disruption
starting 234 seconds after addition of OV (50 mM) (see also Fig. 9). |
download.mov (5 MB)
download.avi (95 MB)
|
movie 2.
Inverse fluorescence microscopy of living OV-treated (10 mM) AK 13-1
cells detecting HK13-EGFP. Images were recorded every 8 seconds and are
presented as averages of consecutive frames. Fine granules are formed
within filaments reaching a maximum 12 minutes after OV addition and
gradually re-integrate into CKFs with almost no thickenings remaining
by 38 minutes (see also Fig. 10). |
download.mov (10 MB)
download.avi (45 MB)
|
movie 3. Time-lapse
fluorescence microscopy of AK 13-1 cells monitoring the distribution of
fusion protein HK13-EGFP after a 3 minute treatment with 10 mM OV.
Pictures were recorded every 15 seconds. A complete CKF network was
rebuild within less than 30 minutes with no remaining granules and a
very homogenous composition (see also Fig. 11). |
download.mov (1,6 MB)
download.avi (11 MB)
|
movie 4. High
magnification of a region taken from movie 3 showing the distribution
of HK13-EGFP in AK13-1 cells after a 3 minute treatment with 10 mM OV
(recording intervals 15 seconds). For best visualization pictures are
shown as averaged consecutive frames. Note the elongation and fusion of
granules and their integration into a fine CKF network. |
